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Throughout these guidelines, quality laboratory science is reinforced by a common-sense approach to biosafety in day-to-day activities.
This scenario can be particularly problematic in laboratories developing new technologies, such as molecular and biochemical technologies, and in point-of-care diagnostics performed by staff unaccustomed to testing that requires biosafety considerations and use of barrier techniques such as personal protective equipment. However, there is a dearth of evidence-based research and publications focused on biosafety; particularly missing are studies documenting safe practices in the day-to-day operations of diagnostic laboratories.
Needle puncture, glass cuts, splash in eye, and bruises and cuts have the highest potential for infection from microbes.
In the hematology laboratory, the major causes of injuries are likely to be exposure to blood and body fluids; needle sticks, aerosols from centrifuge or removal of tube stoppers, tube breakage; or contaminated gloves (22).
In 2008, CDC convened a Blue Ribbon Panel of laboratory representatives from a variety of agencies, laboratory organizations, and facilities to review laboratory biosafety in diagnostic laboratories.
The members of this panel recommended that biosafety guidelines be developed to address the unique operational needs of the diagnostic laboratory community and that they be science based and made available broadly.
LAIs have also included fungal and parasitic infections.
The most common agents of laboratory-acquired fungal infections are the dimorphic fungi Blastomyces, Histoplasma, and Coccidioides (18,19); most reported infections were caused by inhalation of conidia.
Warde Medical Laboratory, Ann Arbor, MI The material in this report originated in the National Center for Emerging and Zoonotic Infectious Diseases, Beth P. Telephone: 678-428-6319; Fax: 770-396-0955; E-mail: [email protected] Summary Prevention of injuries and occupational infections in U. BMBL-5, however, was not designed to address the day-to-day operations of diagnostic laboratories in human and animal medicine.
Michael Miller, Ph D, Microbiology Technical Services, LLC, Dunwoody, GA 30338. CDC and the National Institutes of Health addressed the topic in their publication Biosafety in Microbiological and Biomedical Laboratories, now in its 5th edition (BMBL-5).
This culture of safety is also supported by the Clinical and Laboratory Standards Institute (2). Bureau of Labor Statistics, in 2008, approximately 328,000 medical laboratory technicians and technologists worked in human diagnostic laboratories in the United States.
Work in a diagnostic laboratory entails safety considerations beyond the biological component; therefore, these guidelines also address a few of the more important day-to-day safety issues that affect laboratorians in settings where biological safety is a major focus. An estimated 500,000 persons in all professions work in human and animal diagnostic laboratories.
In a 1986 survey of approximately 4000 workers in 54 public health and 165 hospital laboratories in the United States, 3.5/1000 employee infections occurred in hospital laboratories, and 1.4/1000 employee infections occurred in public health laboratories (15). laboratorians, Neisseria meningitidis accounted for a substantial number of LAIs.